火狐体育

火狐体育多项成果获2020年度市场监管科研成果奖,成为全国唯一获奖高校

来源: 科技处 添加日期:2021-08-28 17:11:56 阅读次数:

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jiexialai,huohutiyukejigongzuozhejiangyixijinpingxinshidaizhongguoteseshehuizhuyisixiangweizhidao,fayangpinbofengxian、yongpangaofengdekexuejingshen,jianjueguanchedangzhongyang、guowuyuanjuecebushu,jianchixinfazhanlinian,shenrushishichuangxinqudongfazhanzhanlve,mianxiangshijiekejiqianyan、mianxiangjingjizhuzhanchang、mianxiangguojiazhongdaxuqiuhemianxiangrenminshengmingjiankang,jiaqianghexinguanjianjishugongguan,jiasukejichengguozhuanhuayingyong,qianghuakejirencaituanduijianshe、jifakejichuangxinhuoli,fenlituijinshichangjianguanxiandaihua,weizhichengshichangjianguanshiyejinbu,fuwujingjishehuifazhanzuochuxindegengdagongxian。

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woguomeinianzhunrushichanghejinjingdechuqinheshuichanpinyue2.3yidun,junxuhegejiance,youqijinqijinkoulenglianshipinxiedaixinguanfeiyanbingdudengdaozhidegaofengxiananquanwenti,jixujianligaofengxianzhibingweishengwudejingzhunshibiehekuaisujiancejishulaibaozhangchuqinheshuichanpindezhilianganquan。muqiancunzaizhezhibingweishengwuweiliangyangbenfujinan、jiancelingmindudi、sihuojunjianbiezhunquelvchadengwenti,yanzhongyingxianglewoguochuqinheshuichanpindezhilianganquanhezhengchangmaoyi。zhenduishangshuwenti,jingshiyunianyanjiu,qudeyixiajishutupohechanpinchuangxin:

1.chuangzhileqiaojutang、suoyijiqiaojutangdengbaofucailiao,tongguobiaomianxiushinamicizhuxianzhuzengjialeqibiaomianfanyinggongnengxingjituanjixifuxingneng;chuangxinzhixiandianjiqudongtuigan+chitiaochilun+chilunzhoubanyuanxiekuaijiegou,shixiancibangtaozidongzhuangxie;shejibingyanzhiledatijiyangbenhe96geyangbentongbuhesuantiquxitong,tiqushijian≤10min,dna huishoulvda 95.86%,hesuantiquyikongjianchacvzhiwei2.9%,shixianlezhibingweishengwuweiliangyangbenhesuangaochunduhegaoxiaolvtiqu。

2.设计荧光探针并构建数据库,计算机辅助循环筛查,创建了高特异性、高扩增效率RPA荧光探针和配对引物的筛查技术,提高多对引物组与荧光探针的兼容性,构建了多重RPA检测技术,检出限<102CFU/mL,检测时间≤15min;探明了LAMP探针标记不同荧光素基团补偿机制,解决了荧光基团相互干扰易淬灭的难题,创建了多靶标同步等温扩增技术,提高了多目标快速检测的准确性和敏感性。

3.通过酪胺信号放大和金标银染两步信号放大技术(TSA-GLSS),结合非荧光标记,创建了高灵敏度和高特异性的致病菌可视化基因芯片检测方法,检测灵敏度达到103copies/mL,检测时间8~10min。

4.探明了致病菌活性与叠氮溴化丙锭(PMA)间的量效关系,明确了PMA在不同致病菌活性检测中的最佳使用浓度,建立了高灵敏致病菌活/死菌PMA-LAMP检测技术,对副溶血弧菌检测限为4.5CFU/mL,霍乱弧菌检测限为4.4CFU/mL,显著降低了假阳性率,与培养法一致。建立的PMA-qPCR检测技术对沙门氏菌的检测限达到 1.0×103CFU/mL。

gongshouquanzhuanli24xiang,qizhongzhongguofamingzhuanli15xiang、guojifamingzhuanli1xiang,shiyongxinxing8xiang;ruanjianzhuzuoquan3xiang;banbuguojiabiaozhun1xiang;fabiaoxueshulunwen27pian,qizhongscishoulu13pian。chengguoyishixianchanyehua,jinsannianleijixiaoshougeleishijihe4.7wanduohe、shizhitiao7000yuwantiao,hesuantiquyi5600duotai,jiancezhuangzhi900duotao,xinzengchanzhi5.45yiyuan,xinzenglishui1.53yiyuan。chanpinzaibeijing、shanghai、guangdong、shandong、zhongqing、liaoning、hubei、zhejiangdengshenghezhixiashideshichangjianguanzhongyingyong,bingbei10yujiashipinjiagonglongtouqiyeyongyujiankongchanpinzhiliangheanquan。zaixinguanfeiyanyiqingqijian,hesuantiquyiyingyongyuhaiguanjinkoulenglianchuqinheshuichanpinjiance,yijiyiyuan、jikongzhongxindengdapilianghesuanjiance,shehuixiaoyixianzhu。chengguoyouxiaotuidongleanquanjiancejishuhechanpindeshengji,baozhanglewoguonongchanpinzhengchangmaoyiheanquan,tishengleguojimaoyihuayuquan。


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